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The effect of MXJDH on macrophage polarization and <t>Pim2/NF-κB</t> pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and <t>Pim2/NF-κB</t> pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and <t>Pim2/NF-κB</t> pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and <t>Pim2/NF-κB</t> pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and <t>Pim2/NF-κB</t> pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and <t>Pim2/NF-κB</t> pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

Journal: Journal of Inflammation Research

Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

doi: 10.2147/JIR.S509734

Figure Lengend Snippet: The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

Article Snippet: Beta-actin rabbit polyclonal antibody (Cat No. 20536-1-AP), PIM2 rabbit polyclonal antibody (Cat No. 25865-1-AP), IL-6 rabbit polyclonal antibody (Cat No. 21865-1-AP), and TNF-α rabbit polyclonal antibody (Cat No. 26162-1-AP) were purchased from Proteintech Biocompany (Hubei, China).

Techniques: Flow Cytometry, Expressing, Co-Immunoprecipitation Assay

MXJDH ameliorates sepsis by inhibiting the Pim2/NF-κB pathway. ( A – E ) Statistical analysis of mRNA expression levels of p65, Pim2, IL-6, IL-10 and TNF-α (n=3). ( F – K ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α and statistical analysis (n=3). ( L ) Protein expression levels of p65 and Pim2 in cytoplasm and nucleus. ( M ) Representative images of P65, Pim2 immunofluorescence staining, and nuclear staining by DAPI. Scale bars, 20 µm as indicated. Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, ****P < 0.0001, VS model group.

Journal: Journal of Inflammation Research

Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

doi: 10.2147/JIR.S509734

Figure Lengend Snippet: MXJDH ameliorates sepsis by inhibiting the Pim2/NF-κB pathway. ( A – E ) Statistical analysis of mRNA expression levels of p65, Pim2, IL-6, IL-10 and TNF-α (n=3). ( F – K ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α and statistical analysis (n=3). ( L ) Protein expression levels of p65 and Pim2 in cytoplasm and nucleus. ( M ) Representative images of P65, Pim2 immunofluorescence staining, and nuclear staining by DAPI. Scale bars, 20 µm as indicated. Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, ****P < 0.0001, VS model group.

Article Snippet: Beta-actin rabbit polyclonal antibody (Cat No. 20536-1-AP), PIM2 rabbit polyclonal antibody (Cat No. 25865-1-AP), IL-6 rabbit polyclonal antibody (Cat No. 21865-1-AP), and TNF-α rabbit polyclonal antibody (Cat No. 26162-1-AP) were purchased from Proteintech Biocompany (Hubei, China).

Techniques: Expressing, Immunofluorescence, Staining

The effect of MXJDH on macrophages in sepsis mice. ( A ) Representative images of F4/80, Pim2 immunofluorescence staining, and nuclear staining by DAPI. Scale bars, 25 µm as indicated. ( B ) Representative images of CD86, CD206 immunofluorescence staining, and nuclear staining by DAPI. Scale bars, 100 µm as indicated.

Journal: Journal of Inflammation Research

Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

doi: 10.2147/JIR.S509734

Figure Lengend Snippet: The effect of MXJDH on macrophages in sepsis mice. ( A ) Representative images of F4/80, Pim2 immunofluorescence staining, and nuclear staining by DAPI. Scale bars, 25 µm as indicated. ( B ) Representative images of CD86, CD206 immunofluorescence staining, and nuclear staining by DAPI. Scale bars, 100 µm as indicated.

Article Snippet: Beta-actin rabbit polyclonal antibody (Cat No. 20536-1-AP), PIM2 rabbit polyclonal antibody (Cat No. 25865-1-AP), IL-6 rabbit polyclonal antibody (Cat No. 21865-1-AP), and TNF-α rabbit polyclonal antibody (Cat No. 26162-1-AP) were purchased from Proteintech Biocompany (Hubei, China).

Techniques: Immunofluorescence, Staining